FIG. 5.

Examination of the involvement of HSV kinases UL13 and US3 in emerin modification. (a) Emerin modification was compared in cells mock infected (M) or infected with wt HSV-1 (strain 17) or a strain 17 deletion mutant lacking UL13 (ΔUL13). Cells were harvested at 16 h p.i. and processed as described in the text. (b) Cells were infected with wt HSV-2 (strain 186), a US3 deletion mutant of strain 186 (ΔUS3), or a rescue mutant of ΔUS3 (US3-R) harvested at 16 h p.i., and emerin modification was examined as described in the text. (c) As controls for the relative progress of infection, lysates were probed with anti-VP5 antibody.