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Comparison of the effect of olomoucine on emerin phosphorylation in the presence or absence of US3-ve cells. Cells were infected (MOI = 10) with HSV-2 (lanes 2 to 5) or HSV2 US3-ve (lanes 6 to 9) strains and incubated with various concentrations (10, 20, or 50 μM) from 4 to 8 h p.i. Cells were then harvested, lysed, and analyzed by Western blotting with anti-emerin antibody.
