FIG. 8.

Altered localization of emerin in HSV-infected cells. Cells plated on coverslips were mock infected (Mock) or infected with HSV-1 (MOI = 10) and at 8 or 16 h p.i., washed in PBS, and fixed directly in 4% PFA (left-hand panels, unextracted) or first extracted with 0.5% TX-100 in the cold prior to fixation with PFA as described in Materials and Methods (right-hand panels, detergent extracted). Endogenous emerin was detected with emerin antibody, and the location was analyzed by laser scanning confocal microscopy using a Zeiss LSM410. Images were obtained by using identical microscope settings in all cases except that, for higher-magnification images of typical cells with corresponding phase-contrast images, an increase in the zoom factor was used in the LSM software.