TABLE 1.
Summary of processed and analyzed tissue sections
| Tissue type | Section thickness (μm) | No. of adjacent tissue sections per slide | Sampling interval (μm) of tissue sections:
|
Avg no. of tissue sections analyzed per infected animalb | |
|---|---|---|---|---|---|
| Infected animalsa | Mock-infected animals | ||||
| Nasal cavity | 5 | 2-4 | 200 | 400 | 74 (coronal), 34 (sagittal) |
| NALTc | 5 | 2-4 | 40 | 100 | 52 (coronal), 15 (sagittal) |
| Lymph nodes | 7 | 4-5 | 56 | 140 | 50 (S), 40 (C), 47 (M) |
| Spleen | 7 | 4 | 56 | 140 | 63 |
| Peyer's patches | 7 | 4 | 56 | 140 | 48 |
| Tongue | 7 | 4 | 252 | 504 | 60 |
a
Maximum distance between tissue sections; the sampling frequency was usually higher so the distance between sections was less.
b
For lymph nodes, the values are subdivided by submandibular (S), cervical (C), and mesenteric (M) lymph nodes.
c
A subset of sections from the nasal cavity that contain NALT.