TABLE 2.
HY TME inoculation of hamsters by the i.c., per os, and e.n. routesa
| Brain inoculum dilutionb | i.c.
|
Per os
|
e.n.
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| Incubation period (days) | No. affected/no. inoculated | Incubation period (days) | No. affected/no. inoculated | Incubation period (days) | No. affected/no. inoculated | ||||
| 10−1 | ND | ND | 158 ± 17 | 4/5 | 161 ± 11 | 5/5 | |||
| 10−2 | ND | ND | 165 ± 10 | 2/5 | 187 ± 39 | 5/5 | |||
| 10−3 | ND | ND | >400 | 0/5 | 224 ± 28 | 4/5c | |||
| 10−4 | ND | ND | ND | ND | 210 | 1/5 | |||
| 10−5 | ND | ND | ND | ND | >400 | 0/5 | |||
| 10−6 | 98 ± 2 | 5/5 | ND | ND | >400 | 0/5 | |||
| 10−7 | 134 ± 9 | 4/5 | ND | ND | >400 | 0/5 | |||
| 10−8 | 192 ± 54 | 3/5 | ND | ND | >400 | 0/5 | |||
| 10−9 | >400 | 0/5 | ND | ND | >400 | 0/5 | |||
| Mock | >400 | 0/5 | >400 | 0/5 | >400 | 0/5 | |||
| Titer | 109.3 | 103.7 | 105.5 | ||||||
a
The titers (calculated by the method of Reed and Muench) for the i.c., per os, and e.n. routes are expressed as the LD50/g. Values are expressed as means ± the standard deviation. ND, not done.
b
Fifty (i.c.) or ten (per os, e.n.) microliters of HY TME brain homogenate was inoculated into each hamster.
c
Significantly greater attack rate compared to per os inoculation (P = 0.03 [Fisher exact test]).