TABLE 1.
Single-cycle infection assay of HTLV-1 vectors encoding various RTs
| Isolatea | Amino acid variation from consensus RT sequence:
|
Relative infectivity (%)b | |||||||
|---|---|---|---|---|---|---|---|---|---|
| H-154 | Q-283 | Q-378 | R-463 | H-472 | L-509 | R-523 | R-533 | ||
| MT-2 | 100 | ||||||||
| C10/MJ | 84 | ||||||||
| Ptnt1 | Q | 135 | |||||||
| Ptnt4 | E | Q | 37 | ||||||
| CH | Y | K | 42 | ||||||
| EG | Q | I | 36 | ||||||
| 3614 | H | Q | I | 37 | |||||
| CS-1 (Q463R) | H | I | Q | 24 | |||||
| CS-1 | H | Q | I | Q | 13 | ||||
a
The RT-encoding pol region of the indicated HTLV-1 isolates and the CS-1 Q463R mutant were cloned into pCMVHT-1Δenv.
b
Normalized to MT-2. The mean of results from duplicate infections was determined for each isolate. CS-1 and MT-2 were tested 10 times. Most other isolates were tested a minimum of three times.