FIG. 7.

The MRN complex does not contribute the effect of IFN on Ad replication. Vero cells were seeded onto coverslips and were untreated (−IFN) (A to E) or pretreated with IFN-α (F to J) or IFN-γ (K to O) for 24 h. Subsequently, the cells were infected with mutant E4inORF3 alone (A, F, and K), dl355-D₁₀₅A/L₁₀₆A alone (B, G, and L), dl355-N₈₂A alone (C, H, and M), or coinfected with E4inORF3 and dl355-D₁₀₅A/L₁₀₆A (D, I, and N) or dl355-N₈₂A (E, J, and O). At 18 h after infection, the cells were immunostained DBP (monoclonal antibody B6-8, Alexa350-coupled secondary antibody) and E4 ORF3 (monoclonal antibody 6A11, TRITC-coupled secondary antibody). The images represent a merge of compressed, deconvolved Z-stacks.