FIG. 2.

Effect of overexpression of green fluorescent protein (GFP)-tagged dominant-negative mutant of Eps15 (D, E, and F or P, Q, and R), Rab 5 (G, H, and I or S, T, and U), or Rab 7 (J, K, and L or V, W, and X) on DNV or WNV infection, respectively. HeLa cells transfected with EGFP-C vector expressing a dominant-negative mutant gene of Eps15 (E95/295) or pQCXIP vector having a dominant-negative Rab 5 or 7 mutant (Rab 5 S34N or Rab 7 T22N) for 30 h was infected with DNV or WNV (MOI of 10) for 16 or 10 h, respectively, and analyzed by immunofluorescence. Control cells were transfected with GFP vector (A, B, and C or M, N, and O). Images were captured by fluorescence microscopy using an ×40 objective lens. Green, either the mutant gene or GFP alone (control); red, virus; blue, nucleus.