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. 2007 Mar 7;81(10):4981–4990. doi: 10.1128/JVI.02361-06

FIG. 3.

FIG. 3.

Demonstration of cross-reactivity between BCoV-DB2 and GiCoV-OH3 by Western blotting with a pool of BCoV-Mebus spike and nucleoprotein MAbs. Lanes: 1, supernatant of HRT-18 cells infected with BCoV-DB2; 2, supernatant of HRT-18 cells infected with GiCoV-OH3; 3, protein ladder (Precision Plus protein standards, dual color; Bio-Rad, Hercules, CA); 4, supernatant of mock-infected HRT-18 cells. *, the smaller size of the band corresponding to the spike protein is due to proteolytic cleavage between the S1 and S2 subunits. The glycosylated S1 subunit represented on the blot has a molecular mass of about 120 kDa.