FIG. 5.

Characterization of the gH2Δ48 mutant. (A) C10 cells were transfected and processed for an immunofluorescence assay (IFA) as described for Fig. 3. The primary antibodies used for detection were as follows: PAb (R176), CHL2 (conformational; group IX), CHL36 (group V), CHL25 (group III), CHL41 (group IV), and CHL29 (group VIII). Those MAbs that were tested but not shown include CHL35 (group V), CHL38 (group VI), and CHL43 (group VII). (B) Schematic of the gH2Δ48 mutant depicting its antigenic regions. Epitopes III through VIII are shown as boxes. Shaded boxes (III, VI, VII, and VIII) indicate epitopes that are presented differently from the WT gH/gL complex, as determined by the IFA used for panel A; white boxes (IV and V) indicate epitopes that are presented in the same way as WT gH/gL. The gH signal sequence (sig) is depicted as a striped box and the transmembrane region (TMR) as a black box. The Δ48 deletion is shown as a gap in the line model.