FIG. 8.

Mutant gH2-L72A/gL is structurally altered compared to gH-WT/gL. (A) C10 cells were transfected and processed for a cell surface immunofluorescence assay as described for Fig. 3. Cells were stained with both the PAb R176 (green) (left) and the anti-gL2 MAb CHL39 (red) (middle). For detection by the conformation-dependent MAb CHL2 (red) (right), the cells were first incubated with the MAb and then subjected to fixation with 3% PFA. (B) Western blot analysis of gH2-WT and the L72A mutant with or without gL2 by using cell lysates of transfected C10 cells. Blots were probed with the anti-gH2/gL2 PAb R176. gH, when coexpressed with gL and then separated on a denaturing gel, runs as a higher-molecular-weight species (top arrowhead) than gH expressed alone (8, 22, 39).