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. 2007 Mar 7;81(10):5155–5165. doi: 10.1128/JVI.01796-06

FIG. 6.

FIG. 6.

Effect of IN putative nuclear localization mutation on RT-IN subcellular localization and particle incorporation. (A and B) Subcellular localization of HIV-1 RT-IN. HeLa cells were transfected with hRN288 (A) or hRN(NLS−) (B); the two are identical except for alanine substitutions for the 186-Lys-Arg-Lys-188 residues in the IN nuclear localization motif. HA-tagged RT-IN proteins were detected as described in the legend for Fig. 5. Bar, 10 μm. (C) Incorporation of HIV-1 RT-IN into VLPs. 293T cells were cotransfected with pGAG and each indicated construct. At 48 h posttransfection, cells and culture supernatant were collected, prepared, and subjected to Western immunoblotting. HA-tagged RT proteins and HIV-1 Gag were probed with anti-HA and anti-p24CA antibodies, respectively.