FIG. 2.

Inhibition of COP-I recruitment to membranes by mutant 3A proteins. (A) Amino acid sequence of CVB3 3A. The C-terminal hydrophobic domain (aa 61 to 82) is depicted in the boxed area. Amino acids that are mutated are indicated by asterisks, and the Ser insertion at position 16 is also indicated. (B to H) BGM cells expressing Myc-tagged wt 3A (B), 3A-REIKI (C), 3A-ins16S (D), 3A-PPP (E), 3A-LL (F), 3A-VDSE (G), and 3A-VREY (H) were stained for the Myc tag and COP-I. (I) Table summarizing the ability of 3A mutants to inhibit protein transport and COP-I recruitment. References in which the mutants are described are indicated. 3A-REIKI, 3A-R6A/E7A/I8A/K9A/I10A; 3A-PPP, 3A-P17A/P18A/P19A; 3A-LL, 3A-L25A/L26A; 3A-VDSE, 3A-V29A/D30A/S31A/E32A; 3A-VREY, 3A-V34A/R35A/E36A/Y37A. Bars, 10 μm.