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. 2007 Feb 28;81(10):5238–5245. doi: 10.1128/JVI.02680-06

FIG. 6.

FIG. 6.

Characterization of the interaction between GBF1 and 3A. (A) Schematic representation of deletion mutants of the N terminus of GBF1. (B) Interaction of 3A with GBF1 deletion mutants in a mammalian two-hybrid system. The firefly luciferase activities measured at 48 h posttransfection are depicted. Average values and standard errors of the means for three experiments are shown. In each experiment, the activity measured with 3A and the intact GBF1 N terminus was set at 100%. (C) Coimmunoprecipitation experiments. 3A-Myc was specifically coprecipitated (IP) from cells cotransfected with YFP-GBF1 but not from cells cotransfected with YFP-GBF1ΔN or Golgi complex-GFP. GBF1-I, aa 1 to 710 of GBF1; GBF1-II, aa 1 to 52 and 295 to 710 of GBF1; GBF 1-III, aa 1 to 392 of GBF1; GBF1-IV, aa 1 to 566 of GBF1; GBF1-V, aa 52 to 710 of GBF1; GBF1ΔN, GBF1 lacking aa 1 to 37. WB, Western blot.