Skip to main content
. 2007 Feb 28;81(10):5246–5256. doi: 10.1128/JVI.02778-06

FIG. 3.

FIG. 3.

(A) Schematic of the 3′RACE strategy employed to detect ambisense S segment vcRNA and mRNA species for NSs and N. (B) Agarose gel results depicting 3′RACE amplification of RVF virus S segment full-length vRNA (left panel, left lane) and full-length vcRNA (left panel, right lane) and NSs and N mRNAs (right panel). Lane L, size marker. (C) Chromatogram sequence data indicating the nucleotide positions of NSs (902) and N (850) mRNA termination. Underlined nucleotides indicate the positions of stop codons for N (915) and NSs (832), with the thin blunt arrows indicating the relative position and direction of each ORF. Note that in each panel the experimentally identified transcription termination signal is boxed and that both NSs and N mRNA species terminate prior to the opposite ambisense ORF. All nucleotide numbering is relative to the virus GenBank entry.