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. 2007 Feb 28;81(10):5246–5256. doi: 10.1128/JVI.02778-06

FIG. 6.

FIG. 6.

(A) Schematic drawing of the wt RVF virus S segment depicting NSs mRNA (left) and N mRNA (right) and the relative position of each putative transcription termination signal. (B) Diagram depicting the deletion of each transcription termination signal, with S-mut 1 containing a deletion of the putative NSs signal s1 and S-mut 2 containing a deletion of the putative N signal s2. (C) Agarose gel results depicting the 3′RACE amplification of vRNA (top left), vcRNA (top right), NSs mRNA (bottom left), and N mRNA (bottom right). Lanes wt, m1, and m2 contain amplification products from wt, S-mut 1, and S-mut 2 viruses, respectively; lane L, size marker. The top right panel indicates that both mutant viruses were able to complete full-length viral complementary S segment replication despite the fact that each putative transcription termination signal was individually removed. Note that the S-mut 1 virus did not generate discrete amplification products for NSs mRNA (bottom left, lane m1) and that the S-mut 2 virus did not generate discrete amplification products for N mRNA (bottom right, lane m2).