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Analysis of Gag and Pol expression and cleavage. 293T cells were transfected with control or viral DNA, and cell lysates as well as viral supernatants were harvested at 2 days posttransfection. (A) Western blot analysis of cell lysates and cell-free virus using polyclonal anti-Gag antiserum. (B) Western blot analysis of cell lysates and cell-free virus using a monoclonal anti-Pol antibody.
