TABLE 2.

Gag expression, particle release, and Gag p68/p71 ratio in wt FV and mutants^a

Virus or construct	Gag expression and particle release (%) inb		p68/p71 ratio in
	293T cells	Viral particles	293T cells	Viral particles
pcDNAc	0	0	0	0
pcHFV	100	100	0.96 ± 0.19	2.15 ± 0.39
RT-IN2	114 ± 25	85 ± 17	0.78 ± 0.17	1.81 ± 0.49
ΔCS	103 ± 21	68 ± 6	0.44 ± 0.11	0.54 ± 0.39
CS2	116 ± 38	109 ± 60	0.97 ± 0.19	1.95 ± 0.60
CS4	76 ± 22	108 ± 11	0.49 ± 0.18	1.18 ± 0.39
CS6	95 ± 19	50 ± 23	0.56 ± 0.21	0.89 ± 0.70
CS8	94 ± 21	58 ± 21	0.41 ± 0.24	0.5 ± 0.43
PR−	73 ± 17	73 ± 11	0	0

^aResults are mean values ± standard deviations from three independent experiments.

^bGag expression and cleavage are normalized to those of wt pcHFV.

^cpcDNA, a plasmid containing a polylinker site (Invitrogen, Carlsbad, CA).