TABLE 3.

Titers of Pol CS mutants after transfection of 293T cells^a

Virus or construct	Infectious units/mlb	% of wild type
pcDNAc	<0.1	<0.1
pcHFV	2.2 (± 0.8) × 106	100.0
RT-IN2	8.3 (± 1.4) × 105	37.7
ΔCS	1.8 (± 1.3) × 104	0.8
CS2	1.4 (± 0.2) × 106	63.6
CS4	4.1 (± 1.4) × 105	18.6
CS6	1.5 (± 0.1) × 105	6.8
CS8	1.7 (± 0.8) × 104	0.8
PR−	<0.1	<0.1
IN−	4.0 (± 6.9) × 105	18.2

^aAssayed on FAB cells.

^bResults are mean values ± standard deviations from three independent experiments.

^cpcDNA, a plasmid containing a polylinker site (Invitrogen, Carlsbad, CA).