TABLE 4.
Titers of Pol CS mutants after infection of HEL cellsa
| Virus or construct | Infectious units/mlb | % of wild type |
|---|---|---|
| pcDNAc | <0.1 | <0.1 |
| pcHFV | 4.6 (± 7.5) × 105 | 100.0 |
| RT-IN2 | 6.0 (± 9.5) × 103 | 1.3 |
| ΔCS | <0.1 | <0.1 |
| CS2 | 5.1 (± 8.6) × 105 | 110.8 |
| CS4 | 1.7 (± 2.2) × 103 | 0.4 |
| CS6 | 2.1 (± 3.2) × 103 | 0.5 |
| CS8 | <0.1 | <0.1 |
| PR− | <0.1 | <0.1 |
| IN− | <0.1 | <0.1 |
a
Assayed on FAB cells.
b
Results are mean values ± standard deviations from three independent experiments.
c
pcDNA, a plasmid containing a polylinker site (Invitrogen, Carlsbad, CA).