FIG. 1.

ICP22 promotes loss of Ser-2P RNAP II in infected cells. (A) Immunoblot analysis of viral IE mutants after CH reversal. Duplicate cultures of Vero cells were mock infected or infected with WT HSV-1 strain KOS1.1 or the mutants indicated. In one set of infections (lanes 1, 3, 5, 7, 9, and 11), the cells were not treated with drugs, and total proteins were harvested at 7 hpi. In the other set (labeled CHR; lanes 2, 4, 6, 8, 10, and 12), cells were infected and maintained in the presence of 100 μg/ml CH for 5 h. The CH was then removed, and the cells were incubated for two more hours prior to harvesting. Protein extracts were analyzed by immunoblotting for Ser-2P RNAP II, EEA1, ICP27, ICP0, ICP4, and ICP22. (B) Analysis of ICP22 and UL13 mutants after CH reversal. The experiment was performed as described for panel A.