FIG. 2.

Loss of Ser-2P RNAP II is ICP22 dependent under IE conditions. Replicate cultures of Vero cells were mock infected or infected with KOS1.1 or d22lacZ. In one set of infections, the cells were left untreated (lanes 1, 4, and 7). In the others, the cells were treated with 100 μg/ml CH for 5 h, followed by 2 h without drugs (labeled CHR; lanes 2, 5, and 8) or 2 h in the presence of 10 μg/ml ActD (labeled CHR/A; lanes 3, 6, and 9). Proteins were harvested from all infections at 7 hpi. (A) Immunoblotting analysis of Ser-2P LS, EEA-1, and ICP8. (B) Quantitation of Ser-2P RNAP II levels. The Ser-2P LS signals from the immunoblot shown in panel A were quantitated by densitometry and normalized to EEA1 levels to correct for protein recovery. The level of Ser-2P RNAP II found in untreated, mock-infected cells (lane 1) was set at 100%.