Skip to main content
. 2007 Mar 7;81(10):5091–5101. doi: 10.1128/JVI.00184-07

FIG. 3.

FIG. 3.

Evidence that ICP22 and an L-gene-dependent factor combine to trigger loss of Ser-2P RNAP II. (A) Effect of PAA on Ser-2P RNAP II loss. Replicate cultures of Vero cells were mock infected or infected with WT HSV-1 or the mutants indicated. In one set of infections, the overlay medium contained 400 μg/ml PAA. Protein extracts were prepared at 8 hpi and analyzed for Ser-2P LS and EEA1 by immunoblotting. (B) Effect of ICP22 and PAA on Ser-2P RNAP II levels in a time-course assay. Replicate cultures of Vero cells were mock infected or infected with WT HSV-1 or d22lacZ in the presence or absence of 400 μg/ml PAA added at 1 hpi. Protein extracts were harvested at the times indicated and analyzed for Ser-2P RNAP II and EEA1 by immunoblotting and densitometry. Ser-2P RNAP II levels were normalized to those for EEA1 to correct for protein recovery. The level of Ser-2P LS found in mock-infected cells was set at 100%.