FIG. 1.

Identification of the off-size fragment covering the junction between Ad12 DNA in TR12 cells (A) and localization of the DNA probes within the Ad12 genome (B). (A) Lane 1, TR12 genomic DNA (30 μg), KpnI and SacI cleavage; lanes 2 and 3, Ad12 DNA (100 pg), KpnI and SacI (lane 2) and KpnI (lane 3) cleavage; M, lambda DNA/Eco130I (StyI) and MluI, marker 17 (MBI Fermentas, Vilnius, Lithuania), 3′ end labeled with [α-³²P]dCTP. DNA was transferred to a positively charged membrane, hybridized to a mixture of probes R, 32, and 28, which were labeled with [α-³²P]dCTP by random priming. (B) Nucleotide numbering of the Ad12 genome is from reference 38. KpnI and SacI sites that are relevant for mapping the integrated DNA are indicated. Filled bars beneath the map of Ad12 DNA show the positions of hybridization probes. Inverted terminal repeats of Ad12 DNA are depicted by white arrowheads. (C) Description of the probes. Probe R was copied from the inverted repeat sequence at the right end of Ad12 DNA; 153 bp (bp 12 to 164 of Ad12 DNA) are homologous to the left end, and 11 bp (bp 165 to 175 of Ad12 DNA) are nonhomologous. The fragments marked with an asterisk were expected to generate off-size bands due to Ad12 termini involved in junctions with cellular DNA or with adjacent Ad12 DNA copies. The inverted terminal repeat of Ad12 DNA is 161 bp (38, 44).