FIG. 2.

Scheme of the Ad12 transgenome and its integration site in cell line TR12. This map is based on a number of restriction endonuclease and Southern blot hybridization experiments as well as partial nucleotide sequence determinations of recloned fragments of the integrated Ad12 DNA. Filled bars beneath the map of the transgenome show the positions of hybridization probes (see also Fig. 1B). Probe C, for the cellular DNA flanking the integrated Ad12 genomes, was excised as a 1,165-bp XbaI fragment from the plasmid subclone F7 (18). Inverted terminal repeats of Ad12 DNA are depicted by white arrowheads. The green bar represents a full-length copy of Ad12 DNA, and the red bar is a truncated second, flip-flopped copy derived from the right terminus of Ad12 DNA. The cellular DNA segments of at least 5.2 kbp adjacent to the transgenome seem to be identical on both flanks and palindromic. Numbers refer to nucleotides in the authentic Ad12 DNA sequence (38) or in the adjacent cellular DNA (18). At the bottom, the magnified structure and sequence of the A-A junction (jagged line) are presented in comparison to the authentic sequence at the 5′ end of Ad12 DNA.