FIG. 5.

HIV-1 antigen-specific CMI responses following pDNA immunization, with and without in vivo EP. Macaques were immunized at 0, 4, and 8 weeks with the three-vector pDNA vaccine design 3c in combination with plasmid rhesus IL-12 by standard i.m. injection (A) or by i.m. injection followed by in vivo EP (B). At various time points, PBLs were collected and tested for HIV-1 Env-, Gag-, Pol-, Nef-, Tat-, and Vif-specific IFN-γ secretion by ELISPOT assay. Bars represent the average total HIV-specific IFN-γ ELISPOT responses (n = 6), with the standard errors of the means indicated. *, statistically significant difference (P < 0.05) between group 3c and group 3cE at the indicated time point.