FIG. 6.

Total HIV-specific CMI responses in CD4⁺ and CD8⁺ cell-depleted PBLs after pDNA immunization alone or in combination with in vivo EP. The graphs show average total HIV peptide pool-specific IFN-γ ELISPOT responses (± standard errors) at week 10 in unfractionated, CD4⁺ or CD8⁺ cell-depleted PBLs from macaques immunized with pDNA vaccines plus plasmid IL-12 alone (A) or followed by in vivo EP (B). Responses were normalized to the precise number of CD3⁺ CD4⁺ or CD3⁺ CD8⁺ cells used in the assay, as determined by flow cytometry after bead depletion. The ratio of the response seen in CD4⁺ cell-depleted PBLs to the response seen in CD8⁺ cell-depleted PBLs is indicated.