TABLE 2.
Persistence of pDNA in injection site DNA and association with HMW DNA
| Group | Macaque no. | BGH-specific primer/probe set
|
HCMV-specific primer/probe set
|
||
|---|---|---|---|---|---|
| pDNA persistencea (plasmid copies/μg injection site DNA) | pDNA association with genomic DNAb (plasmid copies/0.2 μg HMW DNA) | pDNA persistencea (plasmid copies/μg injection site DNA) | pDNA association with genomic DNAb (plasmid copies/0.2 μg HMW DNA) | ||
| 3c | A1N089 | 3,166 | <10 | 129 | <10 |
| A1N097 | 3,682 | <10 | 1,490 | <10 | |
| A2N029 | 918 | 35 | 125 | 34 | |
| A2N042 | 1,388 | <10 | 318 | 36 | |
| A2N060 | 5,101 | <10 | 892 | 23 | |
| A2N070 | 7,206 | <10 | 2,710 | <10 | |
| Mean ± SE | 3,577 ± 958 | 14 ± 4 | 944 ± 414 | 21 ± 5 | |
| 3cE | A1N078 | 507 | <10 | 258 | <10 |
| A1N086 | 217 | <10 | 118 | <10 | |
| A2N010 | 1,601 | <10 | 285 | 15 | |
| A2N014 | 1,043 | <10 | 869 | 13 | |
| A2N036 | 735 | <10 | 165 | <10 | |
| A2N054 | 1,576 | <10 | 89 | 48 | |
| Mean ± SE | 947** ± 231 | 10 | 297* ± 119 | 18 ± 6 | |
| Control | A1N118 | 11,581 | <10 | 5,455 | <10 |
| A2N037 | 1,135 | 13 | 580 | <10 | |
| A2N049 | 16,113 | <10 | 4,103 | 216 | |
| Mean ± SE | 9,610 ± 4,435 | 11 ± 1 | 3,379 ± 1,453 | 79 ± 69 | |
a
The level of vaccine pDNA associated with unpurified injection site tissue DNA was examined 70 days after the final immunization, using a BGH- or HCMV-specific primer-probe pair as described in Materials and Methods. *, significantly reduced (P < 0.05) relative to controls; **, significantly reduced (P < 0.05) relative to group 3c and controls.
b
The level of vaccine pDNA associated with agarose gel-purified HMW genomic DNA was examined 70 days after the final immunization, using a BGH- or HCMV-specific primer-probe pair as described in Materials and Methods.