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. 2007 Mar 7;81(10):5284–5293. doi: 10.1128/JVI.01928-06

FIG. 2.

FIG. 2.

Importin α1, α3, and α5 isoforms interact with Vpr and mediate the nuclear import of Vpr. (A) Digitonin-permeabilized HeLa cells were incubated with 1 μM GST- and GFP-tagged N17C74 and 1 μM of each recombinant importin α isoform. After fixation, cells were analyzed by confocal laser scanning microscopy. Bar = 20 μm. (B) Glutathione-Sepharose beads coupled with 100 pmol GST- and GFP-tagged N17C74 were incubated with 100 pmol recombinant GFP-tagged importin α1, α3, or α5. After incubation for 1 h at 4°C, the bound fractions were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by Coomassie staining. The positions of the three importin α isoforms are indicated.