TABLE 4.
Summary of epitopic domains identified with PTE or CON peptides, in subjects with HIV-1 subtype B infection
| Subject | Presumed epitope/HLA restriction (aa)a | PTE
|
CON
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| Peptide | HXB2 aa position | Sequenceb | SFC/106 PBMC | Peptide no. | HXB2 aa position | Sequence | SFC/106 PBMC | ||
| 1188 | RL9/B7-super (77-85) | 9 | 074-088 | IPLRPMTYKGALDLS | 176 | ||||
| 79 | 074-088 | VPLRPMTYRAARDLS | 313 | ||||||
| TL10/B35 (128-137) | 31 | 126-140 | NYTPGPGTRYPLTFG | 82 | |||||
| 27 | 127-141 | YTPGPGVRYPLTFGW | 492 | ||||||
| UNK | 42 | 175-189 | DPEREVLEWRFDSRL | 53 | |||||
| 1212 | UNK | 64 | 009-023 | SVVGWPAVRERMRRA | 54 | ||||
| UNK | 31 | 126-140 | NYTPGPGTRYPLTFG | 66 | |||||
| UNK | 19 | 160-174 | ENNSLLHPMSLHGMD | 56 | |||||
| 1216 | UNK | 46 | 032-046 | AVSRDLERHGAITSS | 82 | ||||
| FL9/B7 (68-76) | 39 | 063-077 | EEEVGFPVKPQVPLR | 117 | |||||
| UNK | 80 | 188-202 | SLAFHHRARELHPEY | 57 | |||||
| 6 | 190-204 | AFHHMARELHPEYYK | 262 | ||||||
| 40 | 190-204 | AFRHVARELHPEYFK | 167 | ||||||
| 1238 | UNK | 1 | 001-015 | MGGKWSKRSVVGWPT | 53 | ||||
| UNK | 9 | 033-047 | VSRDLEKHGAITSSN | 73 | |||||
| FR10/B7 (68-77) | 17 | 065-079 | EVGFPVRPQVPLRPM | 87 | |||||
| 1362 | UNK | 37 | 108-122 | DILDLWIYHTQGYFP | 1,430 | ||||
| 1484 | FL9/B35/B7 (68-76) | 39 | 063-077 | EEEVGFPVKPQVPLR | 618 | ||||
| 1490 | AK10/A11 (83-92) | 7 | 078-092 | PMTYKAAVDLSHFLK | 60 | ||||
| 3 | 083-097 | GALDLSHFLKEKGGL | 67 | ||||||
| UNK | 2 | 131-145 | PGIRYPLTFGWCFKL | 57 | |||||
| 1576 | UNK | 34 | 101-115 | IYSQKRQDILDLWIY | 67 | ||||
| 1585 | UNK | 80 | 188-202 | SLAFHHRARELHPEY | 57 | ||||
| 52 | 191-205 | FHHKARELHPEYYKD | 73 | ||||||
| 1596 | UNK | 2 | 131-145 | PGIRYPLTFGWCFKL | 462 | ||||
| 1599 | UNK | 1 | 068-082 | FPVRPQVPLRPMTYK | 775 | ||||
| AL8/A2 (84-91) | 3 | 083-097 | GALDLSHFLKEKGGL | 72 | |||||
| UNK | 25 | 090-104 | FLKEKGGLEGLIYSQ | 867 | |||||
| 41 | 090-104 | FLREKGGLEGLVYSQ | 157 | ||||||
| UNK | 27 | 127-141 | YTPGPGVRYPLTFGW | 467 | |||||
| UNK | 46 | 181-195 | LEWKFDSRLAFHHMA | 105 | |||||
| 47 | 185-199 | FDSRLAFHHMARELH | 58 | ||||||
| 1686 | VY8/B35 (74-81) | 9 | 074-088 | IPLRPMTYKGALDLS | 829 | ||||
| 79 | 074-088 | VPLRPMTYRAARDLS | 1,976 | ||||||
| 1690 | UNK | 2 | 131-145 | PGIRYPLTFGWCFKL | 690 | ||||
| EL8/B8 (182-189) | 42 | 175-189 | DPEREVLEWRFDSRL | 227 | |||||
| 35 | 180-194 | VLVWKFDSRLAFRHM | 77 | ||||||
| AY8/A01 (195-202) | 52 | 191-205 | FHHKARELHPEYYKD | 170 | |||||
| 1692 | UNK | 1 | 068-082 | FPVRPQVPLRPMTYK | 101 | ||||
| AL9 (190-198) | 10 | 184-198 | KFDSRLAFHHVAREL | 51 | |||||
| 62 | 184-198 | KF*SRLAFHHIAREKH | 88 | ||||||
| 1693 | WM8/B0801 (13-20) | 4 | 013-027 | WPTVRERMRRAEPAA | 110 | ||||
| UNK | 43 | 052-066 | NAACAWLEAQEEEEV | 193 | |||||
| UNK | 39 | 063-077 | EEEVGFPVKPQVPLR | 1,810 | |||||
| 1 | 068-082 | FPVRPQVPLRPMTYK | 180 | ||||||
a
HLA restriction was inferred based on the subject's known HLA type and published MHC class I restricted CD8+ T-cell epitopes (23), except for epitopes indicated with an asterisk. Optimal epitopes and HLA restrictions for the latter were confirmed using peptide truncations and partially matched and mismatched antigen-presenting cells. UNK, epitope unknown.
b
Amino acid differences from the CON sequence are underlined.