FIG. 2.

Complement is activated during RRV infection. Twenty-four-day-old C57BL/6J wild-type, C3^−/−, or RAG-1^−/− mice were infected with 10³ PFU of RRV by injection in the left rear footpad. At 7 dpi, mice were euthanized, serum was collected, mice were perfused with 1× PBS, and the left ankle or quadriceps muscle was homogenized in radioimmunoprecipitation assay lysis buffer. Immunoblot analyses were performed with polyclonal anti-murine C3 antibody (Cappel) or an anti-α-tubulin antibody as described in Materials and Methods. (A) Serum; (B) ankle tissue; (C) quadriceps muscle tissue.