FIG. 4.

RRV infection in hind limb bone, joint, and skeletal muscle. Twenty-four-day-old C57BL/6J wild-type or C3^−/− mice were infected with 10³ PFU of RRV by injection in the left rear footpad. Mice were sacrificed at 24, 48, 72, 96, and 120 hpi and perfused with 4% paraformaldehyde. After decalcification of calcified tissues, 5-μm-thick paraffin-embedded sections derived from the hind limbs were probed with an ³⁵S-labeled riboprobe complementary either to RRV or to the EBER2 gene from Epstein-Barr virus (control probe). (A) RRV-specific in situ signal in bones and joints of foot at 24 hpi. Two panels per strain showing signal in the periosteum (left panels) or synovial tissue (right panels) are presented. (B) RRV-specific in situ signal in quadriceps muscle tissue.