FIG. 8.

Complement is not required for inflammatory cell recruitment in Ross river virus-infected mice. Twenty-four-day-old C57BL/6J WT and C3^−/− mice were infected with 10³ PFU of RRV by injection in the left rear footpad. (A) At 5, 7, and 10 dpi infiltrating cells were isolated from quadriceps muscle tissue as described in Materials and Methods, and the total cell number was determined. Differences were not statistically different by t test. (B) Cell surface staining of cells isolated from the quadriceps muscle at 7 dpi. Dot plots shown are representative of three mice. Two independent experiments gave similar results. (C) Total numbers of CD11b⁺, NK1.1⁺/CD3⁻, CD3⁺, CD3⁺/CD4⁺, and CD3⁺/CD8⁺ cells isolated from the quadriceps muscle of RRV-infected wild-type (▪) and C3^−/− (□) mice at 7 dpi. The data presented are the means ± the standard errors of the mean of three mice per group and are representative of two independent experiments. *, P < 0.05.