FIG. 1.

Schematic diagram of the Con1 and H77 subgenomic replicons used to measure transient replication efficiency in the Huh-7.5 cell line. The 5′ and 3′ NTR structures are illustrated, and the Con1 and H77 ORFs are depicted as shaded and open boxes, respectively, with the positions of the cell culture-adaptive mutations indicated above the diagrams. Chimeric NS4B proteins are shown below, with open boxes representing H77 NS4B sequences (H) and shaded boxes representing Con1-derived NS4B sequences (C1). The nomenclature used for each replicon is given on the left, and amino acid positions 1, 33, 47, and 261 are indicated below.