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Time course of CRM1 induction during activation of CD4+ T cells. (A) CD4+ T cells isolated from donor 1 and donor 4 were activated with ionophore, PMA, and IL-2 and were analyzed by Western blotting. (B) Time course of CRM1 mRNA induction during activation of CD4+ T cells. CD4+ T cells isolated from donor 1 (□) and donor 4 (⋄) were activated with ionophore, PMA, and IL-2 and were analyzed by quantitative RT-PCR. Each value is the average of duplicate measurements.
