TABLE 1.
SATB1-binding affinities of various HIV-1 integration sequencesa
| Serial no. | DNA clone name | Kd (nM) |
|---|---|---|
| 1 | BH609824 | 10.0 |
| 2 | BH609708 | >50.0 |
| 3 | BH609874 | >40.0 |
| 4 | BH609617 | 5.0 |
| 5 | BH609700 | >40.0 |
| 6 | BH609907 | 5.0 |
| 7 | BH609792 | >50.0 |
| 8 | BH609769 | 4.0 |
| 9 | BH609551 | 8.0 |
| 10 | BH609864 | 15.0 |
| 11 | BH609471 | 2.5 |
| 12 | BH609797 | 15.0 |
| 13 | BH609614 | 60.0 |
| 14 | BH609642 | 10.0 |
| 15 | BH609451 | 15.0 |
| 16 | BH609820 | 25.0 |
| 17 | BH609475 | >40.0 |
| 18 | IgH MAR-WT(25)7 | 1.0 |
a
EMSAs were performed with serial dilutions of recombinant GST-SATB1 as described in Materials and Methods. Relative affinity is depicted as the dissociation constant (Kd; nanomolar concentration). The heptamer of the IgH MAR (16) was used as the reference. For details, see the text.