FIG. 8.

Cyclophilin B knockdown in replicon cells had no significant effect on NS5A expression. (A) Various wild-type and CsA-resistant replicon cells were transduced with lentiviral vectors expressing either a luciferase siRNA (si-Luc) or a CypB siRNA (si-CypB) for 7 days before being lysed for protein analysis. After SDS-PAGE separation, NS5A, CypB, and Ku80 were detected by sequential probing and stripping of these same membranes. (B) Quantitative analysis of the results shown in panel A. After normalization to the loading control Ku80, no specific effect on HCV expression was observed for the CypB knockdown. Error bars indicate standard deviations.