Skip to main content
. 2007 Mar 14;81(11):5607–5616. doi: 10.1128/JVI.02771-06

FIG. 2.

FIG. 2.

Mapping of the interaction of Np9 and Rec with PLZF by GST pull-down assays. (A) GST-Np9 and the indicated GST-Np9 mutants but not GST alone precipitate full-length in vitro translated 35S-labeled PLZF. An outline of the Np9 variants and the PLZF interaction is also shown at bottom. Np9-55mut1 harbors a mutation that incapacitates the NLS1. (B) GST-Rec and the indicated variants bind to radiolabeled PLZF. A scheme summarizing the interactions is given below. NES, nuclear export signal. (C) GST-Np9 but not GST alone precipitates the indicated full-length or truncated variants of in vitro translated 35S-labeled PLZF. (D) GST-Rec precipitates the indicated deletion mutants of radiolabeled PLZF. (E) In vitro coimmunoprecipitation of Np9 and PLZF. The GST-Np9 and PLZF proteins as well as the Np9 binding-defective PLZF(245/543) were transcribed and translated in vitro and coprecipitated with the anti-GST monoclonal antibody 6G9. Note that GST-Np9 fails to bring down PLZF(245/543).