FIG. 7.

CVB3 RNA does not resist or inhibit RNase L activity. (A) ³²P-labeled viral RNAs were assayed as described in the legend to Fig. 1. ³²P-labeled PV (lanes 1 to 4), HCV (lanes 5 to 8), and CVB3 (lanes 9 to 12) RNAs (100 nM) were incubated for 0, 5, 10, or 15 min at 30°C in reaction mixtures containing RNase L and 2-5A (10 nM [each]). RNAs were separated by electrophoresis in 1% agarose and detected by phosphorimaging. (B) ³²P-labeled HCV RNA (100 nM) was incubated at 30°C for 0 (lane 2) or 5 (lanes 3 to 11) min in reaction mixtures containing RNase L and 2-5A (10 nM [each]). The following unlabeled RNAs (0, 100, or 500 nM) were included in the reaction mixtures: PV ORF 2122 (lanes 3 to 5), PV ORF 2121 (lanes 6 to 8), and CVB3 ORF 2122 (lanes 9 to 11). RNA size markers were fractionated in lanes 1 and 12. RNAs from the reactions were separated by electrophoresis in 1% agarose and detected by ethidium bromide staining and UV light or phosphorimaging. The mobility of ORF fragments is indicated with asterisks.