FIG. 1.

Downregulation of S100B expression in BDV persistently infected brain. (A) S100B expression in BDV persistently infected Lewis rat brain. The cerebellum regions of NBI rats were stained with anti-GFAP (red) and anti-S100B (green) antibodies at 5 weeks p.i. The overlap in the distribution of GFAP and S100B is revealed in the merged image. The immunoreaction of S100B is found mainly in the GFAP-positive astrocytes. (B) Expressions of GFAP and S100B were detected in the cerebral cortex and cerebellum persistently infected with BDV. Brain sections from NBI and ABI rats at 5 weeks p.i. were immunostained with anti-GFAP and anti-S100B antibodies. Magnification, ×200. Mock, age-matched mock-infected rats. (C) Expression of S100B and GFAP proteins. The brain homogenates were obtained from the cerebral cortex and cerebellum regions of rat brains at 5 weeks p.i. and subjected to immunoblotting. The quantitative analysis of S100B expression is also shown. The band intensities were determined by NIH image. An n of 6 and n of 3 were used for the statistical analyses of NBI and ABI rats, respectively. Values were normalized to the tubulin level (*, P < 0.05 with mock-infected age-matched control rats).