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. 2007 Mar 28;81(11):6146–6150. doi: 10.1128/JVI.00203-07

FIG. 3.

FIG. 3.

Expression and replication kinetics of Wt and F− viruses in primary cells. (A) Primary CD4+ T cells were costimulated using anti-CD3 and anti-CD28 antibodies in the presence of 20 U/ml interleukin-2 and then infected with denv(Wt) or denv(F−) pseudotype viruses as described for CEM cells in the Fig. 2A legend. Cells were harvested for luciferase assay at the indicated times (mean values ± standard deviations of quadruplicate wells are shown). (B) Stimulated primary CD4+ T cells were infected with Wt or F− replication-competent viruses as described for CEM cells in the Fig. 2C legend, and aliquots of cell-free supernatant were removed at the indicated times for quantification of p24 by enzyme-linked immunosorbent assay. (C) Primary thymocytes were infected with Wt or F− virus and then washed and resuspended in 4 ml of medium containing 20 U/ml interleukin-2 and 20 ng/ml interleukin-4. Cell-free supernatants were removed at the indicated times for quantitation of p24.