FIG. 3.

(A) Effect of mutations of NC protein on the translation inhibition assay. pJC-DB1351, pJC-DB1352, pJC-DB612, and pJC-EJNC6 are described in the text. Data from ONPG assays of wild-type and mutant NC proteins with pMV1/A, a control reporter vector not containing Ψ, and with vector pMV1/A-psi are shown in upper and lower panels, respectively. (B) Interaction strengths of different regions of the Ψ stem-loop sequence with NC using our cell-based assay. Shown are β-galactosidase activities of individual reporter vectors examined in the absence (black bar) and presence (gray bar) of NC. The percentages of lacZ inhibition that indicate β-galactosidase activity obtained with NC compared to that without NC are also shown. All experiments were performed three times.