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. 2007 Mar 21;81(11):6111–6116. doi: 10.1128/JVI.02387-06

FIG. 1.

FIG. 1.

Infectious-clone-derived WNV mutants generated for this study. The organization of the flaviviral genome is shown at the top. 5′, 5′UTR (left shaded box); C, capsid structural protein; 3′, 3′UTR (right shaded box). NS1is followed by NS2A, -2B, -3, -4A, -4B, and -5. The AUG initiation codon and UAG termination codon for the translated polyprotein are indicated. Line drawings representing the genomes of the viruses tested in this study are shown below the genomic map. The Bird 1153 virus isolate contains four amino acid substitutions in the WNV polyprotein (prM-V156I, E-V159A, NS4B-E249G, and NS5-A804V) and four nucleotide substitutions in the 3′UTR (A10596G, C10774U, A10799G, and A10851G), relative to the published NY99 strain sequence (GenBank accession number AF196835). All of these viruses, including the NY99ic virus, contained nucleotide 10851-G. Each Bird 1153 virus-specific locus cloned into the NY99ic background is shown in bold underlined letters for each engineered mutant. 3′UTRB indicates the entire 3′UTRB. The solid triangles indicate the relative position of a conservative NS5-E901D mutation that was engineered to incorporate a SalI site that permitted exchange of the 3′UTRB.