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Env glycoproteins are dispensable for the incorporation of CD40L within HIV-1. NL4-3/CD40L and NL4-3 Env−/CD40L virus stocks were incubated with streptavidin-coated magnetic beads coated with anti-CD40L antibodies (i.e., 5c8) or isotype-matched irrelevant antibodies (i.e., IgG2a). The amounts of precipitated viruses were estimated by a p24 test. The data shown are the means ± standard deviations of triplicate samples and are representative of three independent transfections.
