FIG. 1.

GST-HPV1 E1^E4 coprecipitates SRPK1. (A) Silver-stained 5 to 15% SDS-polyacrylamide gel of GST coprecipitations from lysates prepared from SV-JD keratinocytes showing GST, GST-HPV E1^E4 fusion proteins (lower panel), and associated cellular factors (upper panel). +, lysate present; −, lysate absent. Mass spectrometry analysis by LC MS/MS of trypsin-digested peptides prepared from silver-stained bands identified SRPK1 peptides present in a prominent band (indicated by an asterisk) within the GST-HPV1 E1^E4 coprecipitate. (B) The full-length amino acid sequence of SRPK1 (gene identifier 47419936). Underlined in boldface type are the peptides sequenced from the GST-HPV1 E1^E4 coprecipitation.