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HPV1 E1^E4 associates with Flag-SRPK1 in human keratinocytes. Western analysis of anti-Flag and isotype control immunoprecipitations from lysates prepared from 293T cells coexpressing Flag-SRPK1 and HPV1 E1^E4 or Flag-HPV1 E1 and HPV1 E1^E4. HPV1 E1^E4 was present in immunoprecipitates of Flag-SRPK1 complexes but not in those containing Flag-HPV1 E1, confirming the specificity of the SRPK1-HPV1 E1^E4 association. WB, Western blot; +, present; −, absent.
