FIG. 3.

Immunogenicity of peptides. C57BL/6 (A), C3H (B), or BALB/c (C) mice were immunized s.c. at the base of the tail with 100 μg peptides emulsified in CFA. Draining lymph nodes were taken 7 to 9 days later and LNC (4 × 10⁵/well) incubated with the immunizing peptides or the control peptide NP241. During the last 4 to 8 h of a 3-day culture at 37°C with 5% CO₂, the cells were pulsed with ³H-TdR (0.2 μCi/well), followed by counting of the incorporated radioactivity in a β-counter. Results are expressed as the stimulation index.