FIG. 10.

ORF50+PHIL functions with WT ORF50 transcriptional activity. (A) MBP-50ΔSTAD and MBP-50ΔSTAD+PHIL were analyzed by BN-PAGE as described for Fig. 8A. (B) ORF50+PHIL transactivates transcription with WT ORF50 activity. The indicated plasmids were cotransfected into SLK cells with the K-bZIP/RAP reporter plasmid, extracts were generated, and transactivation was analyzed as described in the legend to Fig. 2A. (C) Equivalent amounts of WT ORF50 and ORF50+PHIL were expressed in transfected SLK cells. Extracts from the experiments for panel A were analyzed by SDS-PAGE/Western blotting, using anti-Rta serum.