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. 2007 Mar 28;81(11):5788–5806. doi: 10.1128/JVI.00140-07

FIG. 14.

FIG. 14.

Tetramerization is required for full ORF50 transcriptional activity. (A) Multimerization of ORF50 chimeric proteins. The indicated proteins were analyzed by BN-PAGE as described in the legend to Fig. 8A. The arrows to the right indicate migration of tetramers and hexamers of MBP-50ΔSTAD and MBP50ΔSTADΔLR, which were included as molecular mass controls. (B and C) Transactivation by ORF50 decreases as the extent of multimerization increases. SLK (B) and 293 (C) cells were transfected with the indicated amounts of plasmids together with the K-bZIP/RAP reporter vector. Extracts were prepared and analyzed as described in the legend to Fig. 2. (D) Equivalent amounts of WT ORF50, 50ΔLR-GCN4, and 50ΔLR-p53TD were expressed in SLK cells. Extracts from the transfection experiment for panel B were analyzed by SDS-PAGE/Western blotting, using anti-Rta primary serum.