FIG. 6.

Infection of LFV and LCMV is not dependent on GAGs. (A) GAG-deficient psgΑ-745 cells and wild-type CHOK1 cells were infected with LFV, LCMV, and VSV pseudotypes at an MOI of 1 or with pseudotypes without GP (no GP). Infection was quantified after 48 h by luciferase assay as described in the legend of Fig. 2F (n = 3 ± SD). (B) Blocking of LFV infection with HS and heparin. Pseudotypes were preincubated with the indicated concentrations of HS and heparin for 1 h on ice and then added to monolayers of Vero cells (MOI of 1). Infection was quantified as described above (A) (n = 3 ± SD). (C) Heparin blocks binding of laminin-1 to α-DG. One microgram of mouse Engelbreth-Holm-Swarm sarcoma laminin-1 per milliliter was preincubated with the indicated concentrations of heparin and then added to purified rabbit skeletal muscle α-DG immobilized in microtiter plates. Bound laminin was detected with a polyclonal anti-laminin-1 antibody, followed by an HRP-conjugated secondary antibody and a color reaction using the substrate ABTS (n = 3 ± SD).